GENETIC-VARIABILITY IN THE SACCHAROMYCES SENSU-STRICTO COMPLEX REVEALED BY MULTILOCUS ENZYME ELECTROPHORESIS

Fifty-two strains of the genus Saccharomyces, including 50 yeasts of t he Saccharomyces sensu stricto complex (sensu Vaughan Martini 1989 [A. Vaughan Martini, Syst. Appl. Microbiol, 12:119-122, 1989]), were anal yzed for nine isozyme loci with starch gel electrophoresis. The geneti c analysis of isozyme data provided further evidence that the Saccharo myces sensu stricto complex is a genetically heterogeneous group, Twen ty-two different electrophoretic types were identified and could be gr ouped into four clusters, However, no correlation between these cluste rs and the currently defined species Saccharomyces cerevisiae, Sacchar omyces bayanus, Saccharomyces pastorianus, and Saccharomyces paradoxus was detected, Several strains could not be assigned to their putative cluster.