DETERMINATION OF XANOMELINE AND ACTIVE METABOLITE, N-DESMETHYLXANOMELINE, IN HUMAN PLASMA BY LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE CHEMICAL-IONIZATION MASS-SPECTROMETRY
At. Murphy et al., DETERMINATION OF XANOMELINE AND ACTIVE METABOLITE, N-DESMETHYLXANOMELINE, IN HUMAN PLASMA BY LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE CHEMICAL-IONIZATION MASS-SPECTROMETRY, Journal of chromatography B. Biomedical applications, 668(2), 1995, pp. 273-280
Citations number
9
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
We have developed a method for the determination of xanomeline and its
pharmacologically active N-desmethyl metabolite. The validated method
uses hexane to extract xanomeline and its N-desmethyl metabolite from
basified plasma. The hexane extract is dried, reconstituted, and anal
yzed using a liquid chromatographic-atmospheric pressure chemical ioni
zation tandem mass spectrometry system. The method was developed to su
pport phase II clinical trials and has proven to be extremely sensitiv
e, fast, and rugged. The method has a limit of quantitation of 75 and
200 pg/ml plasma for xanomeline and the N-desmethyl metabolite, respec
tively. Sample analysis times were less than 3 min from one injection
to the next.