HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF EVODIAMINE IN RAT PLASMA - APPLICATION TO PHARMACOKINETIC STUDIES

A previously published simple and sensitive high-performance liquid ch romatographic method for determination and identification of rutaecarp ine in rat plasma was used for evodiamine determination. However, the ultraviolet detection was not 344 nm, but 227 nm. The method was appli ed to a pharmacokinetic study of evodiamine in rats after 2 mg/kg intr avenous administration. A biphasic phenomenon with a rapid distributio n followed by a slower elimination phase was observed from the plasma concentration-time curve. Compartmental analysis yielded a two-compart ment model.