ACETYLCHOLINESTERASE-BASED AMPEROMETRIC SENSOR

An amperometric sensor has been created using the hydrolysis of acetyl thiocholine iodide, which is catalyzed by the acetylcholinesterase enz yme and generates an electroactive product. An electrochemically modif ied graphite electrode is used, with acetylcholinesterase covalently b onded directly to its surface. The characteristics of the sensor, such as the response time, repeatability, reproducibility, stability, dyna mic range, activation energy, and temperature coefficient of the curre nt are evaluated. The optimum working conditions (pH, temperature, and rotation speed) are established.