PRIMARY STRUCTURE OF A CYTOTOXIN-LIKE BASIC-PROTEIN FROM NAJA-NAJA-NAJA (INDIAN COBRA) VENOM

The complete amino acid sequence of a cytotoxin-like basic protein (CL BP) from the venom of Naja naja naja (Indian Cobra) was determined by manual degradation using a 4-dimethylaminoazobenzene-4'-isothiocyanate double-coupling method. Peptide fragments obtained by chemical cleava ge with cyanogen bromide and enzymic cleavages with trypsin and Staphy lococcus aureus proteases for sequence analysis were purified by rever sed-phase chromatography. The total number of amino acid residues was 61, with leucine as the C-terminal residue. (C) Munksgaard 1995.