ARTIFICIAL CELL-WALL FOR PLANT PROTOPLAST - COATING OF PLASMA-MEMBRANE WITH HYDROPHOBIZED POLYSACCHARIDES

Protoplasts of Daucus carota were coated by a hydrophobized polysaccha ride, such as cholesterol-bearing modified pullulan (CHP) or cholester ol-bearing xyloglucan phosphate (CHXP), that provided an artificial ce ll wall to the protoplast. The polysaccharide-coating was confirmed di rectly by a confocal laser microscopy using FITC-labeled polysaccharid e derivative. The artificial cell wall certainly increased the viabili ty of the protoplasts even in the presence of cellulase.