ENHANCEMENT OF THE ACTIVITY OF BLEOMYCIN BY CYSTEAMINE IN A MICRONUCLEUS ASSAY IN G(0) HUMAN-LYMPHOCYTES

Authors
HOFFMANN GR LITTLEFIELD LG
Citation
Gr. Hoffmann et Lg. Littlefield, ENHANCEMENT OF THE ACTIVITY OF BLEOMYCIN BY CYSTEAMINE IN A MICRONUCLEUS ASSAY IN G(0) HUMAN-LYMPHOCYTES, Toxicology letters, 78(2), 1995, pp. 147-151
Citations number
20
Categorie Soggetti
Toxicology
Journal title
Toxicology letters → ACNP
ISSN journal
03784274
Volume
78
Issue
2
Year of publication
1995
Pages
147 - 151
Database
ISI
SICI code
0378-4274(1995)78:2<147:EOTAOB>2.0.ZU;2-E
Abstract
The aminothiol cysteamine enhances the induction of micronuclei by ble omycin in G(0) human lymphocytes. The potentiation of bleomycin (12.5, 25, 50, or 100 mu g/ml) increased with cysteamine concentration from 5 to 20 mM in a 2-h treatment before culturing the cells for the cytok inesis-block assay. The maximum clastogenic activity of bleomycin in t he presence of cysteamine was more than 10-fold greater than that of t he same dosage of bleomycin alone. Both the thiol and amine functions of aminothiols seem to contribute to the potentiation of bleomycin.