S. Alamanou et G. Doxastakis, THERMOREVERSIBLE SIZE-SELECTIVE SWELLING POLYMERS AS A MEANS OF PURIFICATION AND CONCENTRATION OF LUPIN SEED PROTEINS (LUPINUS-ALBUS SSP GRAECUS), Food hydrocolloids, 9(2), 1995, pp. 103-109
Dilute aqueous protein solutions can be effectively concentrated with
crosslinked thermoreversible size selective polymers of the monomer N-
isopropyl acrylamide using methylene bis-acrylamide. After extracting
the water at 5 degrees C, the gels can be regenerated by warming at 35
degrees C to release the absorbed water and solutes with low molecula
r weight. The resulting separation process is effective for lupin seed
proteins.