THE COWPEA MOSAIC-VIRUS RNA-1-ENCODED 112-KDA PROTEIN MAY FUNCTION ASA VPG PRECURSOR IN-VIVO

Authors
PETERS SA MESNARD JM KOOTER IM VERVER J WELLINK J VANKAMMEN A
Citation
Sa. Peters et al., THE COWPEA MOSAIC-VIRUS RNA-1-ENCODED 112-KDA PROTEIN MAY FUNCTION ASA VPG PRECURSOR IN-VIVO, Journal of General Virology, 76, 1995, pp. 1807-1813
Citations number
33
Categorie Soggetti
Virology,"Biothechnology & Applied Migrobiology
Journal title
Journal of General Virology → ACNP
ISSN journal
00221317
Volume
76
Year of publication
1995
Part
7
Pages
1807 - 1813
Database
ISI
SICI code
0022-1317(1995)76:<1807:TCMR1P>2.0.ZU;2-V
Abstract
Processing of the 112 kDa ('112K') protein encoded by cowpea mosaic vi rus RNA 1 was examined in cowpea mesophyll protoplasts using a transie nt expression system. Cleavage of the 112K protein occurred via two al ternative pathways either into VPg and 110K (24K + 87K) or into 26K (V Pg + 24K) and 87K proteins. The 26K protein can be further cleaved int o VPg and 24K proteins. The results support a model in which the 112K protein functions as the precursor of VPg during initiation of replica tion.