CAPILLARY ELECTROPHORESIS METHODS FOR A CLEAR IDENTIFICATION OF SELENO AMINO-ACIDS IN COMPLEX MATRICES LIKE HUMAN-MILK

Seleno amino acids have been identified in SEC fractions of human milk and quantified by capillary electrophoresis. For this purpose differe nt CE-methods have been developed to separate these seleno amino acids (Se-R-NH3) from other molecules with similar molecular weights. Besid es, methods have been introduced for the exact identification of the a nalytes: These methods allow the identification of Se-R-NH3 in presenc e of molecules of nearly equal mobility and overcome identification pr oblems caused by the shifts of migration times (due to different ionic composition) compared to standard solutions. Procedures for different levels of ''identification securities'' have been ruled out and their ''efficiency'' has been discussed.