INVESTIGATION OF FUNGAL METABOLITES AND ACUTE TOXICITY STUDIES FROM FRUIT-BODIES OF HYPHOLOMA SPECIES (STROPHARIACEAE)

The fruit-bodies of Hypholoma fasciculare and H. capnoides were screen ed by thin-layer chromatography to detect polyols, sugars, phenolic ac ids, alkaloids and fungal metabolites. Mannitol, trehalose, 4-hydroxyb enzoic acid, vanillic acid and choline were mainly observed from both Hypholoma species. The fungal toxins, a-amanitin, bufotenine, muscarin e, muscimol and orellanine were not detected. Acute toxicity studies a nd toxicological investigations were carried out in mice given single intraperitoneal doses of suspensions of the methanol extracts from H. fasciculare and H. capnoides. Paralysis of the respiratory centre, sev ere stomachal ulcers and digestive hemorrhages were revealed with H. f asciculare (LD50 value: 243.29 mg/kg) while no pathology was observed with H. capnoides.