SENSITIVE LASER-INDUCED FLUORESCENCE DETECTION OF POLYAMINE-FLUORESCEINISOTHIOCYANATE-DERIVATIVES AFTER CAPILLARY ZONE ELECTROPHORETIC SEPARATION

Capillary zone electrophoresis (CZE) has been applied for the separati on and quantification of the polyamines putrescine (PUT), cadaverine ( CAD), spermidine (SPD) and spermine (SPM), after their derivatization with the fluoresceinisothiocyanate isomer I (FITC). The derivatization conditions (character of the derivatization medium and concentration, organic modifier, pH and temperature) and CZE operation conditions ha ve been optimized with respect to a sensitive Laser induced fluorescen ce detection (lambda(excitation) = 488nm/lambda(detection) = 520 nm). A complete separation of CAD from PUT was impossible under the conditi ons chosen. The detection limits and relative standard deviations for SPM, SPD, CAD, PUT were determined to be 2.9 (12%), 3.2(7.6%), 0.7(4.6 %), 1.2(7.6%) nmol/L, respectively. The method has been applied to a p ine needle extract.