PEROXIDASE-DEPENDENT FLUORESCENCE DECREASE OF CARBOXYFLUORESCEIN AND ITS ENHANCEMENT BY LIPOSOME ENCAPSULATION - APPLICATION TO THE ENZYMATIC DETERMINATION OF HYDROGEN-PEROXIDE

The fluorescence intensity of 5(6)-carboxy-fluorescein (CF) was decrea sed by addition of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The reaction inside a liposome containing CF and HRP on additi on of H2O2 was measured fluorometrically after destruction of the lipo some with Triton X-100. The reaction efficiency was higher than that w ithout liposome because CF and HRP were concentrated inside the latter . The determination of H2O2 can be performed with a smaller amount of HRP by liposome encapsulation.