Indirect calorimetry is used to assess energy requirements. The Deltat
rac Metabolic Monitor is a relatively inexpensive indirect calorimeter
which uses a 'fixed' flow of ambient air to collect expired air. Only
oxygen and carbon dioxide concentrations are measured and the 'fixed
flow' is assumed in the calculation of oxygen consumption (VO2) and ca
rbon dioxide production (VO2). Using inert gas dilution we have studie
d the effect on VO2, and on the variability in VO2, of changing and le
ngthening the 1.77 m length of 35 mm tubing supplied with the instrume
nt to collect expired air, and of using a mask to collect expired air
instead of the manufacturer's hood. One would anticipate that changing
the tubing could cause a change in resistance to gas flow and thus af
fect the true flow rate. This would alter the gas concentrations seen
by the analysers, but the 'fixed flow' would still be assumed so the r
esults would be in error. Adding extra lengths of manufacturers tubing
caused an apparent rise in VO2 of 0.36%/m of tubing added, and using
22 mm tubing instead of the manufacturer's 35 mm tubing increased VO2
by 0.42% for each 10 cm of tubing added. Using the mask to collect exp
ired air instead of the canopy VO2 was higher, possibly due to the ene
rgy cost of holding the mask, and was more variable, probably because
of poorer mixing of the expired air. To measure VO2 using a mask with
the same precision as a 10 min measurement made with the hood would en
tail measuring VO2 for 14.5 min. The methods used to collect expired a
ir (mask or canopy, length and type of tubing) when measuring metaboli
c rate with the Deltatrac do affect the results obtained but these eff
ects are small and predictable.