ENZYMATIC-SYNTHESIS OF ATP FROM RNA AND ADENINE

The title compound was prepared by a three-stage enzymatic procedure c onsisting of (i) RNA hydrolysis to a mixture of ribonucleosides using intact mycelium of Spicaria violacea, (ii) transribosylation of exogen ous adenine employing whole cells of Escherichia coli as a biocatalyst , and (iii) conversion of formed adenosine into ATP by the enzymes of alcohol fermentation and the kinases extracted from baker's yeast.