RECOMBINANT WHOLE-CELL PENICILLIN ACYLASE BIOCATALYST - PRODUCTION, CHARACTERIZATION AND USE IN THE SYNTHESIS AND HYDROLYSIS OF ANTIBIOTICS

Authors
OSPINA S MERINO E RAMIREZ OT LOPEZMUNGUIA A
Citation
S. Ospina et al., RECOMBINANT WHOLE-CELL PENICILLIN ACYLASE BIOCATALYST - PRODUCTION, CHARACTERIZATION AND USE IN THE SYNTHESIS AND HYDROLYSIS OF ANTIBIOTICS, Biotechnology letters, 17(6), 1995, pp. 615-620
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
6
Year of publication
1995
Pages
615 - 620
Database
ISI
SICI code
0141-5492(1995)17:6<615:RWPAB->2.0.ZU;2-Z
Abstract
The production of a whole cell biocatalyst with high specific penicill in acylase activity using a recombinant strain is described High activ ities were obtained using exponentially fed-batch fermentations to ove rproduce the enzyme. The cells were then entrapped in agar and the act ivity characterized in terms of particle size and,stability. The techn ical feasibility of the catalyst in synthesis and hydrolysis reactions was demonstrated.