IN-VITRO PRODUCTION OF CYCLIC-AMP AND STEROIDS FROM AN OVARIAN SERTOLI-LEYDIG CELL TUMOR NOTES ON CLINICAL MANAGEMENT

A 27 year old nulliparous woman with a history of chronic anovulation and signs of virilization with a markedly elevated serum level of test osterone, underwent a laparotomy with peroperative bilateral ovarian v ein catheterization and bilateral bisection of both ovaries, A solid, 1.5 cm, well delimited tumor located centrally in the right ovary, was excised, Testosterone levels in ovarian venous blood from the tumor b earing side, were 88.4 nmol/l and from the contralateral ovary 3.9 nmo l/l. Histopathological examination showed a Sertoli-Leydig cell tumor which was radically extirpated. Postoperatively, the serum levels of a ndrogen normalized, the woman had regular cycles, became pregnant and delivered a normal female baby. Pieces of tumor tissue were incubated for 2 h, with and without addition of gonadotropins and adrenocorticot ropic hormone (ACTH). Human chorionic gonadotropin (CG)I follicle stim ulating hormone (FSH) and adrenocorticotropic hormone (ACTH) caused si gnificant increases in cyclic monophosphate (cAMP) production in tumor tissue in vitro, as compared to controls. Furthermore, ACTH also sign ificantly stimulated 17 beta-estradiol production.In tumor cells cultu red for 48 h, FSH slightly, but not significantly, increased the produ ction of progesterone. In the cell culture, [H-3]-thymidine incorporat ion into deoxyribonucleic acid (DNA) was stimulated by IGF(1 alpha) bu t not by hCG and FSH. It is concluded that Sertoli-Leydig cell tumors may be sensitive to gonadotropins and ACTH and that their small size, solid shape and intra-ovarian localization can cause diagnostic diffic ulties.