DYSTROPHIN-ASSOCIATED PROTEIN A0 IS A HOMOLOG OF THE TORPEDO 87K PROTEIN

We raised a monoclonal antibody, MAO, which reacts with AO, a 94-kDa r abbit skeletal muscle dystrophin-associated protein (DAP) bound to the syntrophin-binding domain of dystrophin, The antibody also reacted wi th the 62-kDa DAP which was moved to the locus close to beta-syntrophi ns by 2-dimensional PAGE, but the DAP did not coincide with any known beta-syntrophins, We have cloned a fragment of cDNA which codes the pr otein reacting with MAO from a neonatal rabbit heart cDNA library, Bas ed on the coincidence of cDNA sequences and the similarity in molecula r mass, we concluded that the proteins reacting with MAO are rabbit ho mologues of the Torpedo 87K protein.