Kh. Moore et al., SEX-HORMONE BINDING GLOBULIN MESSENGER-RNA IN HUMAN BREAST-CANCER - DETECTION IN CELL-LINES AND TUMOR SAMPLES, Journal of steroid biochemistry and molecular biology, 59(3-4), 1996, pp. 297-304
Sex hormone binding globulin (SHBG) is a high affinity binding protein
for estrogens and androgens. SHBG has been found in breast tissue and
cell lines through immunostaining. The goal of this series of experim
ents was to determine whether mRNA for SHBG is expressed in breast can
cer cell lines and tumor tissue. Reverse transcriptase-polymerase chai
n reaction (RT-PCR) was used to detect SHBG and beta-2 microglobulin (
control for tissue extractions). Three breast cancer cell lines, ZR-75
-1, MCF-7, and MDA-MB-231 and 56 breast tissue samples were collected
and analysed for SHBG mRNA expression. mRNA was successfully extracted
from 30 of these breast tissue samples. SHBG mRNA was detected in ZR-
75-1, MCF-7 and MDA-MB-231 cells, and in 11 of the breast tissue sampl
es. Two PCR products were routinely amplified from the breast cancer c
ell line RNA, one at approximately 500 bp and another at approximately
300 bp. The DNA sequence of the 300 bp PCR produce was consistent wit
h alternate splicing of the SHBG mRNA, where exon 7 is deleted, and is
accompanied by a point deletion at the beginning of exon 8. SHBG prot
ein production from the three breast cancer cell lines was detected by
immunoprecipitation using an affinity purified SHBG antibody. SHBG mR
NA was found in 11 of 30 samples of breast tissue. Some samples expres
sed only the 500 bp or the 300 bp PCR product, whereas others expresse
d both PCR products. The presence of SHBG mRNA in these samples was no
t associated with either the presence or absence of steroid receptors.
SHBG mRNA is thus expressed in breast cancer cell lines, and in some
breast tissue samples. Copyright (C) 1996 Elsevier Science Ltd.