PEROXYNITRITE-INDUCED APOPTOSIS IN HL-60 CELLS

Citation
Kt. Lin et al., PEROXYNITRITE-INDUCED APOPTOSIS IN HL-60 CELLS, The Journal of biological chemistry, 270(28), 1995, pp. 16487-16490
Citations number
33
Categorie Soggetti
Biology
ISSN journal
00219258
Volume
270
Issue
28
Year of publication
1995
Pages
16487 - 16490
Database
ISI
SICI code
0021-9258(1995)270:28<16487:PAIHC>2.0.ZU;2-A
Abstract
Peroxynitrite (ONOO-), an anion and a potent oxidant, generated by the interaction of nitric oxide (NO) and superoxide is able to induce apo ptosis in HL-60 human leukemia cells in a time- and concentration-depe ndent manner. Characteristic morphology of apoptosis can be observed 3 h after HL-60 cells are exposed to 10 mu M ONOO-. Treatment of HL-60 cells with increasing concentrations of ONOO- from 1 to 100 mu M confi rms the concentration dependence of apoptosis as evidenced by: 1) degr adation of nuclear DNA of these cells into integer multiples of approx imately 200 base pairs; 2) colorimetric DNA fragmentation assay; and 3 ) evidence of condensation of chromatin and nuclear fragmentation show n by propidium iodide staining, Under the same conditions, peroxynitri te causes apoptosis in another transformed cell line, U-937 cells, but is ineffective at inducing apoptosis in normal endothelial cells deri ved from human umbilical cord and normal human peripheral blood mononu clear cells, This direct evidence of peroxynitrite inducing apoptosis implicated a new function of this potent oxidant.