IMMUNOREACTIVITY OF NEOPLASTIC AND NONNEOPLASTIC HEPATOCYTES FOR CD68AND WITH 3A5, KI-M1P, AND MAC387 LIGHT-MICROSCOPIC AND ELECTRON-MICROSCOPIC FINDINGS
E. Kaiserling et al., IMMUNOREACTIVITY OF NEOPLASTIC AND NONNEOPLASTIC HEPATOCYTES FOR CD68AND WITH 3A5, KI-M1P, AND MAC387 LIGHT-MICROSCOPIC AND ELECTRON-MICROSCOPIC FINDINGS, HEPATOLOGY RESEARCH, 3(6), 1995, pp. 322-329
The immunohistochemical finding that the macrophage markers KP1, PG-M1
(both CD68) and Ki-M1P occasionally stain epithelial cells prompted a
systematic immunohistochemical investigation of liver tissue with the
se and other macrophage markers. The material investigated comprised n
ormal and cirrhotic liver, four hepatocellular carcinomas, including o
ne fibrolamellar carcinoma, and liver specimens from two cases of extr
ahepatic biliary atresia and one case of paucity of intrahepatic bile
ducts. The macrophage markers KP1, PG-M1, Ki-M1P, 3A5, MAC387 and anti
-lysozyme were employed. Two cases (one each of extrahepatic biliary a
tresia and cirrhosis) were investigated by immunoelectron microscopy t
o determine the subcellular site of reactivity. In the normal liver, o
nly the Kupffer cells reacted with the macrophage markers. However, in
neoplastic and non-neoplastic hepatic disorders, all the antibodies (
with the exception of anti-lysozyme) stained hepatocytes in at least o
ne case. KP1 produced particularly strong staining. Electron microscop
y revealed the reaction product here to be related to lysosome-like gr
anules in the hepatocytes. Therefore, when immunohistochemical investi
gation of liver biopsy specimens reveals reactivity with macrophage ma
rkers, it should be borne in mind that hepatocytes may also be reactiv
e.