The Ultrabithorax (Ubx) gene of the Drosophila bithorax complex is req
uired to specify parasegments 5 and 6. Two P-element ''enhancer traps'
' have been recovered within the locus that contain the bacterial lacZ
gene under the control of the P-element promoter. The P insertion tha
t is closer to the Ubx promoter expresses lacZ in a pattern similar to
that of the normal Ubx gene, but also in parasegment 4 during embryon
ic development. Two deletions have been recovered that remove the norm
al Ubx promoter plus several kilobases on either side, but retain the
lacZ reporter gene. The lacZ patterns from the deletion derivatives cl
osely match the normal pattern of Ubx expression in late embryos and i
maginal discs. The lacZ genes in the deletion derivatives are also neg
atively regulated by Ubx and activated in trans by Contrabithorax muta
tions, again like the normal Ubx gene. Thus, the deleted regions, incl
uding several kilobases around the Ubx promoter, are not required for
long range interactions with Ubx regulatory regions. The deletion deri
vatives also stimulate transvection, a pairing-dependent interaction w
ith the Ubx promoter on the homologous chromosome.