Da. Gdula et Vg. Corces, CHARACTERIZATION OF FUNCTIONAL DOMAINS OF THE SU(HW) PROTEIN THAT MEDIATE THE SILENCING EFFECT OF MOD(MDG4) MUTATIONS, Genetics, 145(1), 1997, pp. 153-161
The suppressor of Hairy-wing [su(Hw)] protein represses enhancer funct
ion in a unidirectional fashion: enhancers segregated from the promote
r by the su(Hw) binding region are rendered inactive, whereas chose in
the same domain are unaffected. In the case of the gypsy-induced y(2)
allele, the repressive effect of su(Hw) is rendered bidirectional in
mod(mdg4)mutant flies, and all enhancers of the affected gene become i
nactive. This silencing of enhancer elements might be due to exposure
of specific domains of su(Hw) when the mod(mdg4) protein is absent. Tw
o of three regions of su(Hw) that are located adjacent to the leucine
zipper motif and are conserved across Drosophila species are necessary
for both the unidirectional and bidirectional repression of transcrip
tion by su(Hw). In contrast, two acidic domains that are dispensable f
or the unidirectional repression of enhancer elements are critical for
the bidirectional silencing of enhancer activity observed in mutants
lacking functional mod(mdg4) protein.