BETA-ADRENERGIC-RECEPTOR SUBTYPE TRANSCRIPTS IN PORCINE ADIPOSE-TISSUE

Expression of transcripts for beta(1)-, beta(2)-, and beta(3)-adrenerg ic receptors (beta(1)-AR, beta(2)-AR, and beta(3)-AR, respectively) wa s investigated in porcine tissues. Northern blot analysis of total RNA indicated hybridization of the rat beta(1)-AR probe to transcripts of approximately 3.0-kb in heart and lung and 2.9-kb in perirenal, oment al, and subcutaneous adipose tissue. Hybridization of the hamster beta (2)-AR probe with total RNA revealed transcripts of approximately 1.8- kb in the heart, 2.0-kb in the lung, and 2.0-kb in perirenal, omental, and subcutaneous adipose tissue; a minor transcript of 1.8-kb was als o observed in the perirenal tissue. Northern blot analysis of poly (A( +)) RNA from porcine adipose tissue with the mouse beta(3)-AR probe re vealed no detectable signals in heart and lung but detected a major 2. 2-kb transcript and a minor 1.9-kb transcript in subcutaneous adipose tissue. The expression of porcine beta(1)-AR and beta(2)-AR transcript s was compared in perirenal, omental, and subcutaneous adipose depots. The expression of beta(1)-AR transcripts was four to five times great er in the perirenal depot than in the omental depot and two to three t imes greater in the subcutaneous depot than in the omental depot. The expression of beta(2)-AR mRNA seemed to be the same in all three depot s.