ENZYME SENSOR FOR L-LACTATE USING DIFFERENTIAL-PULSE AMPEROMETRIC DETECTION

An enzyme sensor using differential pulse (DP) amperometric detection has been developed based on the measurement of the reduction current o f the oxidized form of beta-nicotinamide adenine dinucleotide (NAD(+)) consumed by an enzyme reaction. This biosensor has the definite advan tage to prevent interference caused by electrooxidative species such a s ascorbate and uric acid and exhibits higher sensitivity and selectiv ity in comparison to the classical DC amperometric detector. The linea r detection range of this biosensor was 5.0 x 10(-5)-5.0 x 10(-4) mol/ l and the relative standard deviation (R.S.D.) at 2.5 x 10(-4) mol/l w as 5.0%.