ANALYSIS OF OLIGOSACCHARIDES USING AMINOBONDED SILICA-GEL AND A TERNARY ELUENT WITH EVAPORATIVE LIGHT-SCATTERING DETECTION

Using two commercial, amino bonded, silica gel columns, binary eluents (methanol/water, acetonitrile/methanol) and ternary eluents (acetonit rile/methanol/water) are evaluated for their ability to separate oligo saccharides in comparison with classical acetonitrile/water eluents. C hromatographic data on glucose, maltose, raffinose, stachyose and malt odextrin are reported. Mobile phases consisting of acetonitrile/water or acetonitrile/methanol/water produce the best separations. Phase hyd rolysis is evaluated using an evaporative light scattering detector. T he reduction of the water content in the eluent stabilizes the aminoco lumns and the life time of the amino columns should be improved with t he ternary eluent. Moreover, a better detection limit can be obtained. Capabilities to run gradient elution without baseline drift are clear ly demonstrated.