PURIFICATION AND STABILIZATION OF PHENYLALANINE AMMONIA-LYASE FROM SPORIDIOBOLUS-PARAROSEUS

Authors
MONGE RI LARA M LOPEZMUNGUIA A
Citation
Ri. Monge et al., PURIFICATION AND STABILIZATION OF PHENYLALANINE AMMONIA-LYASE FROM SPORIDIOBOLUS-PARAROSEUS, Biotechnology techniques, 9(6), 1995, pp. 423-428
Citations number
22
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
6
Year of publication
1995
Pages
423 - 428
Database
ISI
SICI code
0951-208X(1995)9:6<423:PASOPA>2.0.ZU;2-L
Abstract
The purification of phenylalanine ammonia-lyase (PAL) from S. pararose us is described: PAL is recovered from cellular extracts by acid preci pitation of contaminating proteins, nucleic acids and carotenoids; con centrated by ultrafiltration and purified by ion exchange chromatograp hy in DEAE-Sephacel, with 80% yield and 32.6 fold purification. Elimin ation of carotenoids and stabilization with glycerol are introduced in the methodology to increase stability and purification yield.