RELEASE OF VIRUS-LIKE PARTICLES BY OSMOTIC SHOCK FROM A MUTANT STRAINOF YEAST DEFICIENT IN CELL INTEGRITY

Authors
ALVAREZ P SANCHEZ M MOLINA M NOMBELA C
Citation
P. Alvarez et al., RELEASE OF VIRUS-LIKE PARTICLES BY OSMOTIC SHOCK FROM A MUTANT STRAINOF YEAST DEFICIENT IN CELL INTEGRITY, Biotechnology techniques, 9(6), 1995, pp. 441-444
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
9
Issue
6
Year of publication
1995
Pages
441 - 444
Database
ISI
SICI code
0951-208X(1995)9:6<441:ROVPBO>2.0.ZU;2-D
Abstract
A yeast system based on a S. cerevisiae mutant strain deficient in cel l integrity, was used to achieve the release of yeast expressed virus- like particles (VLPs) by simple osmotic shock. Yeast cells, grown in t he presence of 1M sorbitol, lysed and released the intracellular conte nt upon being transferred to non-osmotically stabilized medium. The re lease of VLPs was followed by Western blotting determination of the p1 protein.