Adding olive oil to an insect cell (Spodoptera frugiperda) cultivation
with a TNM-FK medium enhanced cell growth. In the static cultivation,
growth with 0.5% oil increased viable cell density by 32%, while cult
ivation in spinner flasks agitated at 260 rpm increased by 64%. With a
gradual increase of agitation from 60 rpm to 500 rpm, the viable eel
density was 81% higher than that without the olive oil supplement.