More and more researchers are interested in imaging living (Henderson,
1994) or fixed cells in their natural environment using the atomic fo
rce microscope (AFM). However, the AFM tip interacts strongly with the
sample, and its z range freedom is limited to a few micrometers. This
means that the cells to be imaged have to be strongly attached to the
substrate, and imaging is restricted to cells having a flattened shap
e. Here we propose a simple and inexpensive solution to overcome these
limitations. The method we propose is trapping living round shaped ce
lls in a Millipore filter with a pore size comparable to the dimension
s of the cell. The highest part of some of the blocked cells protrude
through the holes of the filter and can this way be easily observed us
ing the AFM without detachment.