ANALYSIS OF AMINOBENZOIC ACIDS IN WASTE-WATER FROM A FORMER AMMUNITION PLANT WITH HPLC AND COMBINED DIODE-ARRAY AND FLUORESCENCE DETECTION

Aminobenzoic acids are of major interest in clinical analysis, but the ir determination in environmental samples has been rarely done. Theref ore a method for the determination of 10 aminobenzoic acids in water h as been developed, using high-performance liquid chromatography with c ombined diode array and fluorescence detection. A separation of the an alytes has been achieved with a buffer pH 3/methanol gradient. The gen eral elution order has been m-substituted < p-subst. < o-subst. aminob enzoic acids. UV and fluorescence data are presented. In a highly poll uted water sample of the former ammunition plant Stadtallendorf/Hessen , 4-aminobenzoic acid, 2-aminobenzoic acid and 2-amino-4-nitrobenzoic acid have been detected. After passing a charcoal filter, only the con centration of 4-aminobenzoic acid in a sample from the same site has b een above the detection limit. Besides the ten analytes investigated, several unknown compounds have been present in the samples.