DETERMINATION OF HEMOGLOBIN IN PLASMA AND SERUM BY LINEAR-SWEEP POLAROGRAPHY

A new method for the determination of hemoglobin (Hb) is described. It is based on the amplification of the Hb enzyme-catalyzed reaction bet ween O-phenyldiamine (OPD) and H2O2 and polarographic measurement of t he enzyme-generated product 2,2'-diaminoazobenzene (DAA). A linear cal ibration plot has been obtained within the range of 1 similar to 20 mu g/ml (r = 0.987) Hb. The assay is performed on the microtitre plate t o avoid the influence of serum protein and to obtain good reproducibil ity. The method is practical, reliable and convenient, and has been ap plied to determine Hb in plasma and serum.