GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC IDENTIFICATION OF CHLORINATED OCTADECANOIC ACIDS IN EEL LIPIDS

Gas chromatographic (GC) studies with halogen-selective electrolytic c onductivity detection (ELCD) showed that chlorinated carboxylic acids are major organochlorine compounds in lipids of eel (Anguilla anguilla ) from the receiving waters of a chlorine bleaching sulphite pulp mill , To facilitate the recording of full mass spectra, chlorinated carbox ylic acid methyl esters were cold-trapped after capillary GC separatio n monitored with ELCD, Methyl dichlorooctadecanoate was identified usi ng GC/mass spectrometry (GC/MS) with both electron impact (EI) and amm onia positive-ion chemical ionization (PICI), Methyl tetrachlorooctade canoate was indicated by GC/EIMS, Each chlorinated fatty acid was obse rved at two retention times, suggesting the presence of isomers, The o riginal fatty acid methyl esters derived from the eel lipids were subj ected to GC/MS in the selected-ion monitoring mode; methyl dichlorooct adecanoate was detected using PICI and methyl tetrachlorooctadecanoate was detected using EI ionization.