N. Bahrman et Rj. Petit, GENETIC-POLYMORPHISM IN MARITIME PINE (PINUS-PINASTER AIT) ASSESSED BY 2-DIMENSIONAL GEL-ELECTROPHORESIS OF NEEDLE, BUD, AND POLLEN PROTEINS, Journal of molecular evolution, 41(2), 1995, pp. 231-237
Using two-dimensional polyacrylamide gel electrophoresis, the genetic
variation of proteins was examined in three organs (needle, bud, and p
ollen) from 18 trees of maritime pine. Three types of variation were n
oted: presence/absence, staining intensity, and position variation of
the spots. Of the 902 polypeptides scored in the three organs, 245 (27
.2%) were polymorphic. Moreover, among these variable spots, 117 were
found in a single organ, demonstrating an increased polymorphism of th
e organ-specific polypeptides (56.0% vs 18.4% for the organ-unspecific
polypeptides). Finally, a positive correlation was found between vari
ability level and subunit molecular weight for spots showing position
variation but not for spots showing presence/absence or staining inten
sity variations. Possible explanations for this observation are discus
sed.