GENETIC-POLYMORPHISM IN MARITIME PINE (PINUS-PINASTER AIT) ASSESSED BY 2-DIMENSIONAL GEL-ELECTROPHORESIS OF NEEDLE, BUD, AND POLLEN PROTEINS

Citation
N. Bahrman et Rj. Petit, GENETIC-POLYMORPHISM IN MARITIME PINE (PINUS-PINASTER AIT) ASSESSED BY 2-DIMENSIONAL GEL-ELECTROPHORESIS OF NEEDLE, BUD, AND POLLEN PROTEINS, Journal of molecular evolution, 41(2), 1995, pp. 231-237
Citations number
30
Categorie Soggetti
Genetics & Heredity",Biology
ISSN journal
00222844
Volume
41
Issue
2
Year of publication
1995
Pages
231 - 237
Database
ISI
SICI code
0022-2844(1995)41:2<231:GIMP(A>2.0.ZU;2-B
Abstract
Using two-dimensional polyacrylamide gel electrophoresis, the genetic variation of proteins was examined in three organs (needle, bud, and p ollen) from 18 trees of maritime pine. Three types of variation were n oted: presence/absence, staining intensity, and position variation of the spots. Of the 902 polypeptides scored in the three organs, 245 (27 .2%) were polymorphic. Moreover, among these variable spots, 117 were found in a single organ, demonstrating an increased polymorphism of th e organ-specific polypeptides (56.0% vs 18.4% for the organ-unspecific polypeptides). Finally, a positive correlation was found between vari ability level and subunit molecular weight for spots showing position variation but not for spots showing presence/absence or staining inten sity variations. Possible explanations for this observation are discus sed.