Lr. Garcia et Ij. Molineux, INCOMPLETE ENTRY OF BACTERIOPHAGE-T7 DNA INTO F-PLASMID-CONTAINING ESCHERICHIA-COLI, Journal of bacteriology, 177(14), 1995, pp. 4077-4083
The penetration of bacteriophage T7 DNA into F plasmid-containing Esch
erichia coli cells was determined by measuring Dam methylation of the
entering genome. T7 strains that cannot productively infect F-containi
ng cells fail to completely translocate their DNA into the cell before
the infection aborts. The entry of the first 44% of the genome occurs
normally in an F containing cell, but the entry of the remainder is a
berrant. Bypassing the normal mode of entry of the T7 genome by transf
ecting naked DNA into competent cells fails to suppress F exclusion of
phage development. However, overexpression of various nontoxic T7 1.2
alleles from a high-copy-number plasmid or expression of T3 1.2 from
a T7 genome allows phage growth in the presence of F.