CLONING AND PRIMARY STRUCTURE OF THE CHIA GENE FROM AEROMONAS-CAVIAE

The chiA gene from Aeromonas caviae encodes an extracellular chitinase , 865 amino acids long, that shows a high degree of similarity to chit inase A of Serratia marcescens. Expression in Escherichia coli yielded an enzymatically active protein from which a leader sequence was remo ved, presumably during transport of the enzyme across the cell membran e.