SITE-SPECIFIC RECOMBINATION MEDIATED BY AN ADENOVIRUS VECTOR EXPRESSING THE CRE RECOMBINASE PROTEIN - A MOLECULAR SWITCH FOR CONTROL OF GENE-EXPRESSION

Authors
Citation
M. Anton et Fl. Graham, SITE-SPECIFIC RECOMBINATION MEDIATED BY AN ADENOVIRUS VECTOR EXPRESSING THE CRE RECOMBINASE PROTEIN - A MOLECULAR SWITCH FOR CONTROL OF GENE-EXPRESSION, Journal of virology, 69(8), 1995, pp. 4600-4606
Citations number
49
Categorie Soggetti
Virology
Journal title
ISSN journal
0022538X
Volume
69
Issue
8
Year of publication
1995
Pages
4600 - 4606
Database
ISI
SICI code
0022-538X(1995)69:8<4600:SRMBAA>2.0.ZU;2-I
Abstract
have constructed replication-defective human adenovirus (Ad) type 5 ve ctors containing the gene for the Cre recombinase from bacteriophage P 1 under control of the human cytomegalovirus immediate-early promoter (AdCre). Expression of the protein was detected in replication-permiss ive (293) and in nonpermissive (MRCS) cell lines, and its biochemical activity was demonstrated in a cell-free recombination assay using a p lasmid containing two loxP sites. To study Cre-mediated recombination in an intracellular system, we constructed an Ad vector (AdMA19) conta ining the luciferase cDNA under control of the human cytomegalovirus p romoter but separated from it by an extraneous spacer sequence flanked by loxP sites which blocked luciferase expression. Upon coinfection o f 293 or MRCS cells with AdMA19 and AdCre, luciferase expression was s pecifically induced by Cre-mediated excision of the intervening sequen ce. The use of Ad vectors combined with the Cre-loxP system for regula tion of gene expression and other possible applications is discussed.