SPIN-TRAPPING TECHNIQUE STUDIES ON ACTIVE OXYGEN RADICALS FROM HUMAN POLYMORPHONUCLEAR LEUKOCYTES DURING FLUORIDE-STIMULATED RESPIRATORY BURST

By means of the ESR spectrum technique it was demonstrated that fluori de (F-) stimulated human polymorphonuclear leukocytes (PMN) in a respi ratory burst to generate active oxygen radicals (.O-2- .OH) and to con sume oxygen (O-2). The experiments using the spin trapping technique w ith the nitrone DMPO as the trapping agent indicated that the PMN prod uce the adduct DMPO-OOH under high concentrations of F- and the adduct DMPO-OH under low concentrations of F-. Under medium concentrations o f F-, DMPO-OOH was produced which then decreased in amount as a decrea se occurred in F- concentration. The results of tests of scavenging by superoxide dismutase (SOD) and catalase suggested that the active oxy gen radicals generated in the respiratory burst originated mainly from superoxide (.O-2-). BY the spin probe oximetry method (CTPO and CrOX) it was shown that the O-2 consumed is from the intercellular medium.