APPLICATIONS OF RANDOM PCR

Since the technique of PCR was developed in 1985 (Salk et al.) many ne w forms of applications have been described in the literature. Of part icular interest are PCR approaches, allowing amplification of unknown sequences. In this review these approaches are generally termed ''Rand om PCR''s. While ''conventional PCR'' in the form that was first descr ibed by Saiki et al. is utilized for the amplification and subsequent detection of specific DNA sequences, which are precisely characterized in length and sequence, Random PCR is either used for universal ampli fication of prevailing DNA or for amplification of unknown intervening sequences which are not generally defined in length or sequence. We d epict criteria for discrimination between conventional PCR and Random PCR. Furthermore, we have compiled a classification system for Random PCR approaches based on differentiation by primer structure (degenerat e - non-degenerate). According to this classification system a general overview of published Random PCR approaches is given. Future aspects of application of Random PCR are mentioned and commented. Own investig ations, combining Random PCR with the technique of chromosome microdis section and thus allowing characterization of unknown chromosomes or c hromosome fragments via FISH, are presented. Results are briefly summa rized.