Seed storage protein content of durum wheat (Triticum turgidum var. du
rum) has an important effect on nutritional value and pasta-making cha
racteristics. The objective of this study was to determine by associat
ion with genetic markers the number, chromosomal location, and magnitu
de of effect of quantitative trait loci (QTLs) controlling protein con
centration in kernels. A set of 65 recombinant inbred lines (RIs) was
developed by single seed descent from a cross between cultivated durum
wheat cv. 'Messapia' (low protein content) and accession MG4343 of th
e wild tetraploid wheat var. dicoccoides (high protein content). This
population was characterized for eight morphological, six storage prot
ein, one isozyme and 124 RFLP loci. Field trials were conducted in one
location in 1993 and two locations in 1994. QTLs were mapped by regre
ssion analysis on each marker locus for each location and for the aver
age across environments. A total of six putative QTLs were located on
chromosome arms 4BS, 5AL, 6AS, 6BS and 7BS. The number and size of QTL
s detected varied across environments. The marker with the highest r(2
) value per QTL in each environment and across environments was chosen
for a multiple linear regression analysis, which explained 49.2-56.4%
of the phenotypic variation for protein content. Only some of the mar
kers were found to be negatively associated with plant grain yield and
/or seed weight in one or two of the environments.