TRANSCRIPTION REACTIONS OF YEAST RNA-POLYMERASE-II IN-VITRO

The transcription reactions in vitro of yeast ADH1 and PHO5 gene promo ters are investigated by means of a yeast crude nuclear extract. Using specific RNA probes, the transcription products of these 2 promoters have been first obtained. A low concentration of alpha-amanitin is hig hly inhibitory. The transcription of the PHO5 gene was initiated in vi tro at or near the sites used in vivo. The transcription products incr ease with the amount of the template and reach the maximum at certain concentrations of the template. The deletion of the yeast promoter seq uences abolishes the reaction.