The age determination of red foxes Vulpes vulpes was studied in Pisa P
rovince, Central Italy, from January to May 1992. The presence of incr
emental lines in the cementum of canine teeth after decalcification, s
ectioning and staining has been checked for the first time in a Medite
rranean species. We compared the weight of the eye lens and the width
of pulpar cavity of canine teeth as methods of discriminating yearling
s from adults with the incremental line count (a more reliable, but mo
re time-consuming technique). 330 foxes (125 F; 205 M) were collected,
but sample size was variously reduced for different methods. incremen
tal lines (up to 8) were clearly visible. Neither the eye lens nor the
pulpar cavity methods discriminated unequivocally the two age classes
but a discriminant function using the two variables achieved 100% suc
cess. Pulpar cavity of females closed during their first oestrus. Geog
raphical variability in the development rates of different organs sugg
ests that no method (except incremental lines) can be used without pri
or verification.