THE HUMAN GENE ENCODING THE LARGEST SUBUNIT OF RNA-POLYMERASE-II

The nucleotide sequence of a large portion of the human RNA polymerase II large subunit (RpII LS)-encoding gene and its whole gene structure were determined, The RpIILS gene consists of 29 exons. The sequence o f the 5' flanking region is highly conserved as compared with that of the mouse RpIILS and contains several SP1-binding sites, a CCAAT seque nce and a sequence homologous to a heat-shock element, In addition, se veral inverted repeats and palindrome sequences were involved in the 5 ' upstream region, Those suggest that the 5' flanking domain of RpIILS would be highly structured which may be responsible for transcription al regulation.