IMPROVED CONDITIONS FOR SOLID-PHASE SYNTHESIS OF OLIGONUCLEOTIDES ON PS-PEG COPOLYMERS

Polystyrene-polyethylene glycol (PS-PEG) tentacle polymers with loadin gs of up to 60 mu mol/g were used for standard oligonucleotide synthes is, As these resins are easy to handle and stable under reaction and c leavage conditions they may be used alternatively to controlled pore g lass (CPG) as the most commonly used solid support for oligonucleotide synthesis. However, structural and chemical properties of the PS-PEG resins require modified conditions to guarantee syntheses with high co upling efficiencies. Oligonucleotides (ODN) of various sequences and l engths have successfully been synthesized using HPLC and capillary ele ctrophoresis (CE) for purity control. Additionally, electrospray mass spectrometry (ES-MS) was used for product identification.