E. Bayer et al., IMPROVED CONDITIONS FOR SOLID-PHASE SYNTHESIS OF OLIGONUCLEOTIDES ON PS-PEG COPOLYMERS, Zeitschrift fur Naturforschung. B, A journal of chemical sciences, 50(7), 1995, pp. 1096-1100
Polystyrene-polyethylene glycol (PS-PEG) tentacle polymers with loadin
gs of up to 60 mu mol/g were used for standard oligonucleotide synthes
is, As these resins are easy to handle and stable under reaction and c
leavage conditions they may be used alternatively to controlled pore g
lass (CPG) as the most commonly used solid support for oligonucleotide
synthesis. However, structural and chemical properties of the PS-PEG
resins require modified conditions to guarantee syntheses with high co
upling efficiencies. Oligonucleotides (ODN) of various sequences and l
engths have successfully been synthesized using HPLC and capillary ele
ctrophoresis (CE) for purity control. Additionally, electrospray mass
spectrometry (ES-MS) was used for product identification.