Jl. Smilanick et al., EVALUATION OF HEATED SOLUTIONS OF SULFUR-DIOXIDE, ETHANOL, AND HYDROGEN-PEROXIDE TO CONTROL POSTHARVEST GREEN MOLD OF LEMONS, Plant disease, 79(7), 1995, pp. 742-747
Lemon fruit were inoculated with spores of Penicillium digitatum and i
mmersed in solutions of ethanol, sulfur dioxide, or hydrogen peroxide
to control postharvest green mold. Green mold incidence and fruit inju
ry were assessed after treatments employing various combinations of co
ncentration, duration of treatment, temperature, and post-treatment ri
nses. Heating of the solutions was needed to attain acceptable efficac
y. Sulfur dioxide and ethanol controlled green mold without injury to
fruit, whereas hydrogen peroxide did not effectively control green mol
d and caused unacceptable injury to fruit. Treatments selected for ext
ensive evaluation were immersion in 10% ethanol at 45 degrees C for 15
0 s without rinsing, or in 2% sulfur dioxide at 45 degrees C for 150 s
followed by two fresh water rinses. These treatments were compared wi
th two existing decay control methods: immersion in 3% sodium carbonat
e at 45 degrees C for 150 s followed by two fresh water rinses, or in
1,000 mu g/ml imazalil at 25 degrees C for 60 s. Lemons were inoculate
d at 20 degrees C then incubated for 12, 24, 48, or 60 h before treatm
ents were applied. Efficacy of sulfur dioxide and ethanol treatments w
as comparable to that of sodium carbonate and imazalil. Sulfur dioxide
and ethanol did not injure the fruit and their residues were low. The
sulfur dioxide content of lemons immediately after treatment was less
than 1 mu g/ml. The ethanol content of lemons analyzed immediately af
ter ethanol treatment was 58.6 (+/-9.6) mu g/ml and 24.4 (+/-11.7) mu
g/ml after storage for 7 days at 20 C. The ethanol content of untreate
d fruit was 3.3 mu g/ml.