EXPRESSION AND LOCALIZATION OF THE CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR MESSENGER-RNA AND ITS PROTEIN IN RAT-BRAIN

In previous studies we have characterized the expression of the cystic fibrosis transmembrane conductance regulator (CFTR) protein in clathr in-coated vesicles derived from bovine brain and in neurons of rat bra in. In this study we have further characterized the expression of the CFTR protein mRNA and protein in rat brain with reverse transcriptase polymerase chain reaction amplification (RT-PCR), in situ hybridizatio n, and immunocytochemistry, The expression of CFTR mRNA and protein in discrete areas of brain, including tbe hypothalamus, thalamus, and am ygdaloid nuclei, which are involved in regulation of appetite and rest ing energy expenditure, is identical. The presence of CFTR in neurons localized to these regions of brain controlling homeostasis and energy expenditure may elucidate the pathogenesis of other nonpulmonary and gastrointestinal manifestations which commonly are observed in childre n with cystic fibrosis. Dysregulation of normal neuropeptide vesicle t rafficking by mutant CFTR in brain may serve as a pathogenic mechanism for disruption of homeostasis.