IMMUNOCYTOCHEMICAL LOCALIZATION OF ALLERGENIC PROTEIN (OLE-E-I) IN THE ENDOPLASMIC-RETICULUM OF THE DEVELOPING POLLEN GRAIN OF OLIVE (OLEA-EUROPAEA L)

The monoclonal antibody OL-1 and transmission electron microscopy were used to locate immunologically the major allergen of olive pollen. Ol e e I, during pollen grain development. Within the pollen grain, aller genic proteins are located in the cisternae of the rough endoplasmic r eticulum. Our findings indicate that the synthesis of these proteins s tarts in the vegetative cytoplasm of olive pollen during the early mat uration stage.