RESTRICTED INTRAMOLECULAR ENERGY-FLOW IN THE ENZYME-SUBSTRATE COMPLEX

The fate of the energy released during the formation of the enzyme-sub strate complexes is discussed in connection with energetic aspects of the one-substrate enzyme-catalysed reaction. Assuming a free and rapid intramolecular energy flow in the complex, the high enzyme catalytic activity cannot be explained. it is suggested that a metal ion near th e active site can serve as a barrier to the energy flow from the bindi ng sites of the complex into the enzyme molecule. The effective vibrat ional temperature of the bonded substrate molecule is then higher than the temperature of the reaction system. (C) 1995 Academic Press Limit ed