EVALUATION OF A SIMPLIFIED HEP-2 CELL ADHERENCE ASSAY FOR ESCHERICHIA-COLI ISOLATED FROM SOUTH INDIAN CHILDREN WITH ACUTE DIARRHEA AND CONTROLS

Authors
KANG G MATHAN MM MATHAN VI
Citation
G. Kang et al., EVALUATION OF A SIMPLIFIED HEP-2 CELL ADHERENCE ASSAY FOR ESCHERICHIA-COLI ISOLATED FROM SOUTH INDIAN CHILDREN WITH ACUTE DIARRHEA AND CONTROLS, Journal of clinical microbiology, 33(8), 1995, pp. 2204-2205
Citations number
12
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
8
Year of publication
1995
Pages
2204 - 2205
Database
ISI
SICI code
0095-1137(1995)33:8<2204:EOASHC>2.0.ZU;2-B
Abstract
A simplified HEp-2 cell adhesion assay was performed with stored Esche richia coli isolates from 761 children with acute diarrhea and 531 mat ched controls, and the results were evaluated by means of fluorescent actin staining and hybridization with DNA probes. The prevalence rates of localized adherence and aggregative adherence mere significantly h igher for patients (9.9 and 7.6%, respectively) than for Controls (3.7 and 3.9%, respectively).