IMMUNOCYTOCHEMICAL STUDY OF HUMAN PROLACT IN RECEPTORS USING ANTIIDIOTYPIC ANTIBODIES IN HUMAN BREAST-CANCER

In this study, we have prepared antiidiotypic antibodies specific of h uman prolactin receptors (PRL-R) in order to localize these receptors in breast cancer. Antiidiotypic antibodies were prepared using anti-hu man prolactin (anti-hPRL) sera obtained from New Zealand rab-bits. 25 breast cancer were PRL-R positive using radio receptor assay. For immu nohistochemistry, the tissus fixed with Nakane and treated by NH4Cl an d Saponine to improve staining specificity, were incubed with antiidio typic antibodies (1/100(e)). Cytoplasm and membran of human breast can cer cells were PRL-R positive in 20/25 breast cancer with antiidiotypi c antibodies. There was no staining with preimmune serum and immunohis tochemical staining elimination by prior absorption with anti-hPRL ant ibodies.